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[Construction of middle fragment deletion mutant with improved gene splicing by overlap extension].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Feb 2004

Li Chen-hua, Fang Hai-yan, Deng Xiao-yun, Xia Kun, Zheng Duo, Xia Jia-hui

Abstract excerpt

OBJECTIVE: To construct a phosphatidylinositol 4-kinase beta (PI4K-beta) mutant with the 325th to 373rd amino acid codons deleted, and try to develop a simple method for constructing middle fragment deletion mutant. METHODS: In line with the mechanism of gene splicing by overlap extension(SOE), an additional PCR was used to get the PI4K-beta mutant in which the 325th to 373rd amino acid codons were deleted. Then...

Topics

1-Phosphatidylinositol 4-Kinase
Base Sequence
Genetic Vectors
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Polymerase Chain Reaction
Protein Engineering
Recombinant Proteins
Sequence Deletion

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PMID: 14767910

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