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Gene cloning and seamless site-directed mutagenesis using single-strand annealing (SSA).

Applied microbiology and biotechnology - 1 Dec 2018

Luo Zhixin, Wang Shanhe, Jiao Beilei, Yuan Dan, Dai Dongmei, Wang Lixian, Xu Kun, Wang Xin

Abstract excerpt

The full length of interested genes can be usually cloned by assembling exons or RACE products through overlap PCR. However, the procedure requires multiple PCR steps, which are prone to random mutagenesis. Here, we present a novel SSA-based method for gene cloning and seamless site-directed mutagenesis. We firstly cloned the full-length coding sequence of Cashmere goat (Capra hircus) Hoxc13 gene by assembling...

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PMID: 30209551
DOI: 10.1007/s00253-018-9348-z

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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