Constructing recombinant DNA molecules by PCR.

doi:10.1002/0471142727.mb0317s78

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Constructing recombinant DNA molecules by PCR.

Current protocols in molecular biology - 1 Apr 2007

Elion Elaine A, Marina Pablo, Yu Lu

Abstract excerpt

This unit describes the use of PCR to construct hybrid DNA molecules. The unit provides an overview of how PCR can be exploited to accomplish numerous cloning strategies. The Basic Protocol outlines the PCR amplification and cloning strategies. The Commentary includes a troubleshooting guide for problems most frequently encountered in PCR cloning, plus four specific examples of the application of this technique...

Topics

DNA Restriction Enzymes
DNA, Recombinant
Genetic Vectors
Mutation
Plasmids
Polymerase Chain Reaction
Recombinant Fusion Proteins
Saccharomyces cerevisiae

Source

Open in PubMed

Identifiers

PMID: 18265404
DOI: 10.1002/0471142727.mb0317s78

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics