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Inexpensive and easy method for 6 fragment Golden Gate Assembly of a modular S/MARs mammalian expression vector and its variants

2021-09-04

Abstract excerpt

<h4>Background</h4> A basic requirement for synthetic biology is the availability of efficient DNA assembly methods. Numerous methods have been previously reported to accomplish this task. One such method has been reported, which allows parallel assembly of multiple DNA fragments in a one-tube reaction, called Golden Gate Assembly. This study aims to further simplify that method and make it more suitable for smal...

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Literature Corpus work
91556dc6-0b32-58c5-b142-ee9e5f16bb00
DOI
10.1101/2021.09.04.458594
Open publication

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Inexpensive and easy method for 6 fragment Golden Gate Assembly of a modular S/MARs mammalian expression vector and its variantsDOI 10.1101/2021.09.04.458594
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