Article

Massively parallel DNA target capture using Long Adapter Single Stranded Oligonucleotide (LASSO) probes assembled through a novel DNA recombinase mediated methodology

2021-05-11

Abstract excerpt

In the attempt to bridge the widening gap from DNA sequence to biological function, we developed a novel methodology to assemble Long-Adapter Single-Strand Oligonucleotide (LASSO) probe libraries that enabled the massively multiplexed capture of kilobase-sized DNA fragments for downstream long read DNA sequencing or expression. This method uses short DNA oligonucleotides (pre-LASSO probes) and a plasmid vector tha...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 45323f7b-643d-5193-b95b-8629853ae3ff
DOI: 10.22541/au.162075978.89906964/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Massively parallel DNA target capture using Long Adapter Single Stranded Oligonucleotide (LASSO) probes assembled through a novel DNA recombinase mediated methodologyDOI 10.22541/au.162075978.89906964/v1Read

A strategy for genome-wide seamless tagging of human protein-coding genesSemantic proximity Read

Multigene amplification and massively parallel sequencing for cancer mutation discovery.Semantic proximity Read

<i>De novo</i> assembly, delivery and expression of a 101 kb human gene in mouse cellsSemantic proximity Read

Multiplex amplification of large sets of human exons.Semantic proximity Read

Development of Coupling Controlled Polymerizations by Adapter-ligation in Mate-pair Sequencing for Detection of Various Genomic Variants in One Single AssaySemantic proximity Read

Detecting Full-Length EccDNA with FLED and long-reads sequencingSemantic proximity Read

A versatile 5′ RACE-Seq methodology for the accurate identification of the 5′ termini of mRNAsSemantic proximity Read

Large-scale discovery of recombinases for integrating DNA into the human genomeSemantic proximity Read

Barcoded oligonucleotides ligated on RNA amplified for multiplex and parallel in-situ analysesSemantic proximity Read

A Multiplexed Amplicon Approach for Detecting Gene Fusions by Next-Generation SequencingSemantic proximity Read

Fluorescently guided workflow with rationally engineered 5′ ligation adapters for high-sensitivity and low-bias small RNA sequencingSemantic proximity Read

FLASH assembly of TALENs for high-throughput genome editing.Semantic proximity Read