Article
In-fusion assembly: seamless engineering of multidomain fusion proteins, modular vectors, and mutations.
BioTechniques - 1 Sept 2007
Zhu Baogong, Cai Guifang, Hall Emily O, Freeman Gordon J
Abstract excerpt
In-Fusion can join any two pieces of DNA that have a 15-bp overlap at their ends. The result is equivalent to a recombination event at the ends of the DNAs. The 15-bp overlap may be engineered by inclusion in primers used to PCR amplify a segment of DNA. Originally described for inserting one piece of DNA into a restriction enzyme-digested plasmid, we have found In-Fusion can join four or more pieces of DNA in a...
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