Rapid and precise engineering of the Caenorhabditis elegans genome with lethal mutation co-conversion and inactivation of NHEJ repair.

Abstract excerpt

As in other organisms, CRISPR/Cas9 methods provide a powerful approach for genome editing in the nematode Caenorhabditis elegans. Oligonucleotides are excellent repair templates for introducing substitutions and short insertions, as they are cost effective, require no cloning, and appear in other organisms to target changes by homologous recombination at DNA double-strand breaks (DSBs). Here, I describe a...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to DNA End-Joining Repair
• Share to Gene Conversion
• Share to Gene Knock-In Techniques