Article

Optimized iMAP CRISPR Perturbation Illuminates the Context-Specific Functions of RNA Modification Factors

2025-07-27

Abstract excerpt

iMAP is a large-scale CRISPR perturbation platform based on a germline-transmitted transgene comprising individually floxed, tandemly linked guides that can be inducibly expressed via Cre-mediated recombination. A key limitation was strong recombination bias favoring the proximal guides. Here, we effectively mitigated this bias by inactivating a recombination hotspot, yielding guide representation nearly as unifor...

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Identifiers and source

Literature Corpus work: 478a1903-441c-5af7-89b5-b41dfbccbb5a
DOI: 10.1101/2025.07.24.666689

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