Article

Spatially Resolved <i>in vivo</i> CRISPR Screen Sequencing via Perturb-DBiT

2024-11-19

Abstract excerpt

<h4>SUMMARY</h4> Perturb-seq enabled the profiling of transcriptional effects of genetic perturbations in single cells but lacks the ability to examine the impact on tissue environments. We present Perturb-DBiT for simultaneous co- sequencing of spatial transcriptome and guide RNAs (gRNAs) on the same tissue section for in vivo CRISPR screen with genome-scale gRNA libraries, offering a comprehensive understanding...

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Literature Corpus work: 12a42183-3ffe-5f19-916b-855e46ebdfdb
DOI: 10.1101/2024.11.18.624106

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