Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

CRISPR-Cas9 Genome Editing in Human Cell Lines with Donor Vector Made by Gibson Assembly.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

Sahoo Nirakar, Cuello Victoria, Udawant Shreya, Litif Carl, Mustard Julie A, Keniry Megan

Abstract excerpt

CRISPR Cas9 genome editing allows researchers to modify genes in a multitude of ways including to obtain deletions, epitope-tagged loci, and knock-in mutations. Within 6 years of its initial application, CRISPR-Cas9 genome editing has been widely employed, but disadvantages to this method, such as low modification efficiencies and off-target effects, need careful consideration. Obtaining custom donor vectors can...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Line
Share to Clustered Regularly Interspaced Short Palindromic Repeats

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CRISPR-Associated Protein 9

Share to CRISPR-Cas Systems

Share to Forkhead Box Protein O3

Share to Gene Editing

Share to Genetic Vectors

Share to RNA, Guide, CRISPR-Cas Systems

Source

Open in PubMed Open full text

Identifiers

PMID: 32006411
DOI: 10.1007/978-1-0716-0290-4_20
PMCID: PMC7391466

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

CRISPR-Cas9 Genome Editing in Human Cell Lines with Donor Vector Made by Gibson Assembly.PMID 32006411Read ↗

CRISPR/Cas9-mediated genome editing: From basic research to translational medicine.Semantic proximity Read ↗

Genome editing: A perspective on the application of CRISPR/Cas9 to study human diseases (Review)Semantic proximity Read ↗

Clinical applications of the CRISPR/Cas9 genome-editing system: Delivery options and challenges in precision medicineSemantic proximity Read ↗

Current approaches in CRISPR-Cas9 mediated gene editing for biomedical and therapeutic applications.Semantic proximity Read ↗

Advancements in CRISPR/Cas systems for disease treatmentSemantic proximity Read ↗

CRISPR-Cas systems for genome editing of mammalian cells.Semantic proximity Read ↗

An introduction to CRISPR-Cas systems for reprogramming the genome of mammalian cells.Semantic proximity Read ↗

Recent Progress in CRISPR/Cas9 Technology.Semantic proximity Read ↗

All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells.Semantic proximity Read ↗

New advances in CRISPR/Cas-mediated precise gene-editing techniques.Semantic proximity Read ↗

Progress and Perspective of CRISPR‐Cas9 Technology in Translational MedicineSemantic proximity Read ↗

CRISPR/Cas9 a simple, inexpensive and effective technique for gene editingSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.