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Article

Multiple sgRNAs with overlapping sequences enhance CRISPR/Cas9-mediated knock-in efficiency.

Experimental & molecular medicine - 6 Apr 2018

Jang Da Eun, Lee Jae Young, Lee Jae Hoon, Koo Ok Jae, Bae Hee Sook, Jung Min Hee, Bae Ji Hyun, Hwang Woo Sung, Chang Yoo Jin, Lee Yoon Hoo, Lee Han Woong, Yeom Su Cheong

Abstract excerpt

The CRISPR/Cas9 system is widely applied in genome engineering due to its simplicity and versatility. Although this has revolutionized genome-editing technology, knockin animal generation via homology directed repair (HDR) is not as efficient as nonhomologous end-joining DNA-repair-dependent knockout. Although its double-strand break activity may vary, Cas9 derived from Streptococcus pyogenens allows robust...

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Share to Clustered Regularly Interspaced Short Palindromic Repeats
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29622782
DOI: 10.1038/s12276-018-0037-x
PMCID: PMC5938013

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