Article

Optimizing Heterologous Production of CRISPR-AsCas12a Protein in Escherichia coli

2024-07-15

Abstract excerpt

<title>Abstract</title> <p>The CRISPR-Cas12a system is a groundbreaking tool that has seen an ample use for genome editing and diagnostics in biotechnology and biomedicine research labs. Despite its increasing use, there is a lack of studies on optimizing Cas12a protein production at lab-scale using straightforward protocols. This study aimed on enhancing the lab-scale recombinant production of <italic>Acidaminoc...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: f9a2f8e2-9c13-57c6-a146-3d129796f534
DOI: 10.21203/rs.3.rs-4535821/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Optimizing Heterologous Production of CRISPR-AsCas12a Protein in Escherichia coliDOI 10.21203/rs.3.rs-4535821/v1Read

Programmable and portable CRISPR-Cas transcriptional activation in bacteriaSemantic proximity Read

A Combinatorial Approach towards Adaptability of 22 Functional Cas12a Orthologs for Nucleic Acid Detection in Clinical SamplesSemantic proximity Read

Uses and Advantages of CRISPR/Cas Genetic Edition in YeastsSemantic proximity Read

CRISPR-Cas tools for simultaneous transcription & translation control in bacteriaSemantic proximity Read

Expanding the CRISPR toolbox by engineering Cas12a orthologs of metagenomic discoverySemantic proximity Read

Engineering Cas9: next generation of genomic editors.Semantic proximity Read

NmeCas9 is an intrinsically high-fidelity genome editing platformSemantic proximity Read

An update on CRISPR-Cas12 as a versatile tool in genome editing.Semantic proximity Read

Directed evolution expands CRISPR-Cas12a genome editing capacitySemantic proximity Read

Fusion of a non-specific DNA-binding domain enhances Cas12a trans-cleavage for robust nucleic-acid diagnosticsSemantic proximity Read

Repurposing CRISPR-Cas12b for mammalian genome engineeringSemantic proximity Read

Applications of Anti-CRISPR Proteins in Genome Editing and BiotechnologySemantic proximity Read