Article

Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

2018-06-08

Abstract excerpt

<h4>ABSTRACT</h4> Spatial/temporal control of Cas9 guide RNA expression could considerably expand the utility of CRISPR-based technologies. Current approaches based on tRNA processing offer a promising strategy but suffer from high background. Here we developed a variant screening platform to identify differential sequence determinants of human tRNA promoter and processing activities. Rational design based on the...

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Literature Corpus work: 89b200a2-69f4-56ca-8560-c44f741c0d94
DOI: 10.1101/342485

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