Article

Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells

2021-05-14

Abstract excerpt

RNA-targeting CRISPR-Cas13 proteins have recently emerged as a powerful platform to transiently modulate gene expression outcomes. However, protein and CRISPR RNA (crRNA) delivery in human cells can be challenging and knockdown can be transient due to rapid crRNA degradation. Here we compare several chemical RNA modifications at different positions to identify synthetic crRNAs that improve RNA targeting efficiency...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: bf7900ad-4859-5cfe-8163-f12360d43af3
DOI: 10.1101/2021.05.12.443920

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cellsDOI 10.1101/2021.05.12.443920Read

Chemically Modified Cpf1-CRISPR RNAs Mediate Efficient Genome Editing in Mammalian Cells.Semantic proximity Read

A Cas9 nanoparticle system with truncated Cas9 target sequences on DNA repair templates enhances genome targeting in diverse human immune cell typesSemantic proximity Read

Generation of knock-in primary human T cells using Cas9 ribonucleoproteinsSemantic proximity Read

Principles for rational Cas13d guide designSemantic proximity Read

Ribonucleoprotein Transfection for CRISPR/Cas9‐Mediated Gene Knockout in Primary T CellsSemantic proximity Read

Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity.Semantic proximity Read

RNA-programmed genome editing in human cellsSemantic proximity Read

Synthetic CRISPR RNA-Cas9–guided genome editing in human cellsSemantic proximity Read

Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cellsSemantic proximity Read

The CRISPR-Cas12a Platform for Accurate Genome Editing, Gene Disruption, and Efficient Transgene Integration in Human Immune CellsSemantic proximity Read

Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cellsSemantic proximity Read

Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteinsSemantic proximity Read