Article

Massively parallel assessment of human variants with base editor screens

2020-05-17

Abstract excerpt

Understanding the functional consequences of single-nucleotide variants is critical to uncovering the genetic underpinnings of diseases, but technologies to characterize variants are limiting. Here we leverage CRISPR-Cas9 cytosine base editors in pooled screens to scalably assay variants at endogenous loci in mammalian cells. We benchmark the performance of base editors in positive and negative selection screens a...

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Identifiers and source

Literature Corpus work: 4161a069-ebf7-59ab-84ee-febf20be4634
DOI: 10.1101/2020.05.17.100818

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