Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Massively parallel assessment of human variants with base editor screens.

Cell - 18 Feb 2021

Hanna Ruth E, Hegde Mudra, Fagre Christian R, DeWeirdt Peter C, Sangree Annabel K, Szegletes Zsofia, Griffith Audrey, Feeley Marissa N, Sanson Kendall R, Baidi Yossef, Koblan Luke W, Liu David R, Neal James T, Doench John G

Abstract excerpt

Understanding the functional consequences of single-nucleotide variants is critical to uncovering the genetic underpinnings of diseases, but technologies to characterize variants are limiting. Here, we leverage CRISPR-Cas9 cytosine base editors in pooled screens to scalably assay variants at endogenous loci in mammalian cells. We benchmark the performance of base editors in positive and negative selection...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to bcl-X Protein
Share to BRCA1 Protein

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to BRCA2 Protein

Share to Catalytic Domain

Share to Gene Editing

Share to Loss of Function Mutation

Share to Myeloid Cell Leukemia Sequence 1 Protein

Share to Poly(ADP-ribose) Polymerases

Source

Identifiers

PMID: 33606977
DOI: 10.1016/j.cell.2021.01.012

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Massively parallel assessment of human variants with base editor screens.PMID 33606977Read ↗

Massively parallel assessment of human variants with base editor screensSemantic proximity Read ↗

Benchmarking of SpCas9 variants enables deeper base editor screens of <i>BRCA1</i> and <i>BCL2</i>Semantic proximity Read ↗

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors.Semantic proximity Read ↗

Annotation and evaluation of base editing outcomes in multiple cell types using CRISPRbase.Semantic proximity Read ↗

High-throughput screening of human genetic variants by pooled prime editingSemantic proximity Read ↗

High-throughput screening of human genetic variants by pooled prime editingSemantic proximity Read ↗

Mapping functional elements of the DNA damage response through base editor screens.Semantic proximity Read ↗

A side-by-side comparison of variant function measurements using deep mutational scanning and base editingSemantic proximity Read ↗

Activity-based selection for enhanced base editor mutational scanningSemantic proximity Read ↗

Decoding disease-relevant variants with base and prime editors at scale.Semantic proximity Read ↗

Functional interrogation of DNA damage response variants with base editing screens.Semantic proximity Read ↗

Base-editing mutagenesis maps alleles to tune human T cell functions.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.