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Article

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors.

Journal of visualized experiments : JoVE - 28 Feb 2021

See Ji-Eun, Shin Ha Rim, Jang Gayoung, Kweon Jiyeon, Kim Yongsub

Abstract excerpt

Recent studies have investigated the risks associated with BRCA1 gene mutations using various functional assessment methods such as fluorescent reporter assays, embryonic stem cell viability assays, and therapeutic drug-based sensitivity assays. Although they have clarified a lot of BRCA1 variants, these assays involving the use of exogenously expressed BRCA1 variants are associated with overexpression issues and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33720123
DOI: 10.3791/61557

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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