Article

CRISPR-Cas guided mutagenesis of chromosome and virulence plasmid in <i>Shigella flexneri</i> by cytosine base editing

2022-03-05

Abstract excerpt

Shigella is a Gram-negative bacterium that invades the human gut epithelium. The resulting infection, shigellosis, is the deadliest bacterial diarrheal disease. Much of the information about the genes dictating the pathophysiology of Shigella , both on the chromosome and the virulence plasmid, was obtained by classical reverse genetics. However, technical limitations of the prevalent mutagenesis techniques restri...

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Literature Corpus work: 25c585f4-3142-564f-a4d8-31085105b580
DOI: 10.1101/2022.03.04.482438

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