Article

Cas9-mediated endogenous plasmid loss in <i>Borrelia burgdorferi</i>

2022-09-22

Abstract excerpt

<h4>ABSTRACT</h4> The spirochete Borrelia burgdorferi , which causes Lyme disease, has the most segmented genome among known bacteria. In addition to a linear chromosome, the B. burgdorferi genome contains over 20 linear and circular endogenous plasmids. While many of these plasmids are dispensable under in vitro culture conditions, they are maintained during the natural life cycle of the pathogen. Plasmid-enco...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: c80b36d2-cd88-5302-a8a1-ad531f3d06f9
DOI: 10.1101/2022.09.22.509095

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Cas9-mediated endogenous plasmid loss in <i>Borrelia burgdorferi</i>DOI 10.1101/2022.09.22.509095Read

Inducible CRISPRi-based operon silencing and selective <i>in trans</i> gene complementation in <i>Borrelia burgdorferi</i>Semantic proximity Read

A CRISPR interference platform for selective downregulation of gene expression in <i>Borrelia burgdorferi</i>Semantic proximity Read

High-Throughput Plasmid Content Analysis of <i>Borrelia burgdorferi</i> B31 by Using Luminex Multiplex TechnologySemantic proximity Read

The plasmids of Borrelia burgdorferi: essential genetic elements of a pathogenSemantic proximity Read

Mosaic Plasmids are Abundant and Unevenly Distributed Across Prokaryotic TaxaSemantic proximity Read

CRISPR-Cas guided mutagenesis of chromosome and virulence plasmid in <i>Shigella flexneri</i> by cytosine base editingSemantic proximity Read

Lost in plasmids: next generation sequencing and the complex genome of the tick-borne pathogen Borrelia burgdorferiSemantic proximity Read

[Bacterial plasmids].Semantic proximity Read

<i>Streptococcus pyogenes</i> Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in <i>Trypanosoma</i> and <i>Leishmania</i>Semantic proximity Read

pGGTOX: a versatile plasmid platform for recombination-based genome editing across <i>Enterobacteriaceae</i>Semantic proximity Read

Experimental assessment of the roles of linear plasmids lp25 and lp28-1 of Borrelia burgdorferi throughout the infectious cycle.Semantic proximity Read

Distinct plasmid- and host-encoded mechanisms drive small plasmid copy number-mediated heteroresistance in <i>Escherichia coli</i>Semantic proximity Read