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Mobile-CRISPRi as a tool for genetic manipulation in the intracellular pathogen <i>Piscirickettsia salmonis</i>

2025-08-06

Abstract excerpt

Piscirickettsia salmonis is the causative agent of Salmonid Rickettsial Septicemia (SRS), the main bacterial disease affecting the salmon industry in Chile. In this work, we implemented a Mobile-CRISPRi system to generate gene silencing using a catalytically inactive dCas9 protein and an IPTG-inducible single-guide RNA (sgRNA). We demonstrate the efficacy of the CRISPRi system in P. salmonis by silencing an exoge...

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Identifiers and source

Literature Corpus work
8fb80753-1bc1-5c67-8f41-895b95f69b6f
DOI
10.1101/2025.08.05.668709
Open publication

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