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Article

SCRaMbLE-in: A Fast and Efficient Method to Diversify and Improve the Yields of Heterologous Pathways in Synthetic Yeast.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

Swidah Reem, Auxillos Jamie, Liu Wei, Jones Sally, Chan Ting-Fung, Dai Junbiao, Cai Yizhi

Abstract excerpt

The synthetic chromosome rearrangement and modification by LoxP-mediated evolution (SCRaMbLE) system is a key component of the synthetic yeast genome (Sc2.0) project, an international effort to construct an entire synthetic genome in yeast. SCRaMbLE involves the introduction of thousands of symmetrical LoxP (LoxPsym) recombination sites downstream of every nonessential gene in all 16 chromosomes, enabling...

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PMID: 32809206
DOI: 10.1007/978-1-0716-0908-8_17

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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