Article
A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity.
Biotechnology letters - 1 May 2020
Larroude Macarena, Trabelsi Heykel, Nicaud Jean-Marc, Rossignol Tristan
Abstract excerpt
OBJECTIVES: The construction and validation of a set of Yarrowia lipolytica CRISPR/Cas9 vectors containing six different markers that allows virtually any genetic background to be edited, including those of wild-type strains. RESULTS: Using the Golden Gate method, we assembled a set of six CRISPR/Cas9 vectors, each containing a different selection marker, to be used for editing the genome of the industrial yeast...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
