Article

Conserved and species-specific STIM autoinhibition revealed by AlphaFold3-guided dissection

2026-07-27

Abstract excerpt

<h4>SUMMARY</h4> Store-operated Calcium (Ca 2+ ) entry (SOCE), mediated by dynamic STIM–Orai coupling, is crucial for cellular signaling, yet the molecular architecture of STIM autoinhibition remains poorly defined. Using an AlphaFold3-guided functional validation strategy combining FRET-based biosensors, mutagenesis, and Ca 2+ imaging, we systematically dissected the CC1–SOAR autoinhibitory interface in both h...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 3f34392e-5bc0-5299-b76f-0955593d05ab
DOI: 10.64898/2026.07.27.740881

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Conserved and species-specific STIM autoinhibition revealed by AlphaFold3-guided dissectionDOI 10.64898/2026.07.27.740881Read

Dissecting gating mechanisms of Orai calcium channel paralogs using constitutively active Orai mutants that mimic STIM1-gated stateSemantic proximity Read

Orai channel pore properties and gating by STIM: implications from the Orai crystal structure.Semantic proximity Read

Intramolecular shielding maintains the ER Ca²⁺ sensor STIM1 in an inactive conformation.Semantic proximity Read

Comprehensive mutational characterization of the calcium-sensing STIM1 EF-hand reveals residues essential for structure and function.Semantic proximity Read

Comprehensive mutational characterization of the calcium-sensing STIM1 EF-hand reveals residues essential for structure and functionSemantic proximity Read

Discovery of novel gating checkpoints in the Orai1 calcium channel by systematic analysis of constitutively active mutants of its paralogs and orthologs.Semantic proximity Read

STIM1/Orai1 coiled-coil interplay in the regulation of store-operated calcium entry.Semantic proximity Read

Sequential activation of STIM1 links Ca2+ with luminal domain unfolding.Semantic proximity Read

High affinity associations with α-SNAP enable calcium entry via Orai1 channels.Semantic proximity Read

STIM1 GoF Mutants: Genotype-Phenotype Relationships Across the Stormorken/TAM/YPS Spectrum.Semantic proximity Read

CRAC channel opening is determined by a series of Orai1 gating checkpoints in the transmembrane and cytosolic regions.Semantic proximity Read

Molecular determinants of the coupling between STIM1 and Orai channels: differential activation of Orai1-3 channels by a STIM1 coiled-coil mutant.Semantic proximity Read