Article

Enhancing Precise Genome Editing in Human Pluripotent Stem Cells through Dual Inhibition of DNA Damage Response and Repair Pathways

2023-06-21

Abstract excerpt

<title>Abstract</title> <p>Precise genome editing is crucial for establishing isogenic human disease models and <italic>ex vivo</italic> stem cell therapy from the patient-derived human pluripotent stem cells (hPSCs). Unlike Cas9-mediated knock-in, cytosine base editor (CBE) and prime editor (PE) achieve the desirable gene correction without inducing DNA double strand breaks. However, hPSCs possess highly active...

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Literature Corpus work: 3cbd1d73-9b48-5b5d-8d22-2e11b2e101a9
DOI: 10.21203/rs.3.rs-3028152/v1

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