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Article

Mitochondrial pH monitored by a new engineered green fluorescent protein mutant.

The Journal of biological chemistry - 19 Mar 2004

Abad María F Cano, Di Benedetto Giulietta, Magalhães Paulo J, Filippin Luisa, Pozzan Tullio

Abstract excerpt

We here describe a new molecularly engineered green fluorescent protein chimera that shows a high sensitivity to pH in the alkaline range. This probe was named mtAlpHi, for mitochondrial alkaline pH indicator, and possesses several key properties that render it optimal for studying the dynamics of mitochondrial matrix pH, e.g. it has an apparent pK(a) (pK(a)') around 8.5, it shows reversible and large changes in...

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Identifiers

PMID: 14701849
DOI: 10.1074/jbc.M306766200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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