Article

One-step induction of human GABAergic neurons promotes presynaptic development & synapse maturation

2025-07-04

Abstract excerpt

Human induced pluripotent stem cells (iPSCs) present a powerful approach to study human brain physiology and disease, yet robust, pure GABAergic induction has remained difficult. Here we present improved, single-step, transposon-based GABAergic induction with Ascl1/Dlx2, which yields, unlike lentiviral approaches, exclusively GABAergic neurons and was validated across three independent iPSC lines. Co-seeding with...

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Literature Corpus work: 360a5797-b711-5b41-8ab6-94a8fc1a1c5c
DOI: 10.1101/2025.06.30.662293

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