Article

Single-Molecule Analysis of DNA-binding proteins from Nuclear Extracts (SMADNE)

2022-04-01

Abstract excerpt

Single-molecule characterization of protein-DNA dynamics provides unprecedented mechanistic details about numerous nuclear processes. Here, we describe a new method that rapidly generates single-molecule information for fluorescently tagged proteins isolated from nuclear extracts of human cells. This approach determines binding lifetimes ( k off ), events per second (∼ k on ), positional dependence (specificity)...

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Literature Corpus work: 308f3d58-e2f4-5281-92a3-1233c8835a09
DOI: 10.1101/2022.04.01.486290

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