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Single-molecule live imaging of subunit interactions and exchange within cellular regulatory complexes

23 Jul 2025

Abstract excerpt

Cells are built from vast networks of interdependent molecular interactions. Here, we combine proximity-assisted photoactivation (PAPA) with automated fast single-molecule tracking (fSMT) to probe subunit interactions within endogenous protein complexes in live human cells. PAPA-fSMT revealed that the inactive positive transcription elongation factor b (P-TEFb):7SK ribonucleoprotein complex is predominantly...

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PMID: 40706597
DOI: 10.1016/j.molcel.2025.06.028

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