Article

The translation inhibitor cycloheximide affects ribosome profiling data in a species-specific manner

2019-08-24

Abstract excerpt

Ribosome profiling provides genome-wide snapshots of translation dynamics by determining ribosomal positions at sub-codon resolution. To maintain this positional information, the translation inhibitor cycloheximide (CHX) has been widely used to arrest translating ribosomes prior to library preparation. Several studies have reported CHX-induced biases in yeast data casting uncertainty about its continued use and qu...

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Literature Corpus work: 307ccec8-57f8-50c6-aa88-ab45c682fd7b
DOI: 10.1101/746255

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