Article

Streamlined and sensitive mono- and diribosome profiling in yeast and human cells

2023-02-03

Abstract excerpt

Ribosome profiling has unveiled diverse regulations and perturbations of translation through a transcriptome-wide survey of ribosome occupancy, read out by sequencing of ribosome-protected mRNA fragments. Generation of ribosome footprints and their conversion into sequencing libraries is technically demanding and sensitive to biases that distort the representation of physiological ribosome occupancy. We address th...

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Literature Corpus work: 069a26fd-9764-5f4a-982e-86f991eb315d
DOI: 10.1101/2023.02.01.526718

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