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An enhanced Eco1 retron editor enables precision genome engineering in human cells without double-strand breaks

2024-08-05

Abstract excerpt

Retrons are a retroelement class found in diverse prokaryotes that can be adapted to augment CRISPR-Cas9 genome engineering technology to efficiently rewrite short stretches of genetic information in bacteria and yeast. However, efficiency in human cells has been limited by unknown factors. We identified non-coding RNA (ncRNA) instability and impaired Cas9 activity due to 5' sgRNA extension as key contributors to...

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Literature Corpus work
304359a1-f6c4-5dc4-b8c2-736dfcb672c4
DOI
10.1101/2024.08.05.606586
Open publication

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An enhanced Eco1 retron editor enables precision genome engineering in human cells without double-strand breaksDOI 10.1101/2024.08.05.606586
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