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Retron Editing for Precise Genome Editing without Exogenous Donor DNA in Human Cells

2021-05-11

Abstract excerpt

CRISPR-Cas9 mediated seamless genome editing can be achieved by incorporating donor DNA into the CRISPR-Cas9 target loci via homology-directed repair (HDR), albeit with relative low efficiency due to the inefficient delivery of exogenous DNA. Retrons are bacterial genetic element composed of a non-coding RNA (ncRNA) and reverse transcriptase (RT). Retrons coupled with CRISPR-Cas9 have been shown to enhance precise...

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Literature Corpus work
2cfbb9bc-89b8-5f24-ac04-e3dbeb7930c5
DOI
10.1101/2021.05.11.443596
Open publication

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Retron Editing for Precise Genome Editing without Exogenous Donor DNA in Human CellsDOI 10.1101/2021.05.11.443596
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