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Bacterial retrons enable precise gene editing in human cells

2021-03-29

Abstract excerpt

Retrons are bacterial genetic elements involved in anti-phage defense. They have the unique ability to reverse transcribe RNA into multicopy single-stranded DNA (msDNA) that remains covalently linked to their template RNA. Retrons coupled with CRISPR-Cas9 in yeast have been shown to improve editing efficiency of precise genome editing via homology-directed repair (HDR). HDR editing efficiency has been limited by c...

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Literature Corpus work
63c1d3b9-b46f-54d7-aa53-2979f62eaccf
DOI
10.1101/2021.03.29.437260
Open publication

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Bacterial retrons enable precise gene editing in human cellsDOI 10.1101/2021.03.29.437260
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