Article
Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure.
Nature communications - 29 Mar 2022
Li Xiaosa, Zhou Lina, Gao Bao-Qing, Li Guangye, Wang Xiao, Wang Ying, Wei Jia, Han Wenyan, Wang Zixian, Li Jifang, Gao Runze, Zhu Junjie, Xu Wenchao, Wu Jing, Yang Bei, Sun Xiaodong, Yang Li, Chen Jia
Abstract excerpt
Prime editor (PE), which is developed by combining Cas9 nickase and an engineered reverse transcriptase, can mediate all twelve types of base substitutions and small insertions or deletions in living cells but its efficiency remains low. Here, we develop spegRNA by introducing same-sense mutations at proper positions in the reverse-transcription template of pegRNA to increase PE's base-editing efficiency up-to...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.