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Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated transcriptome in human cells

2024-03-08

Abstract excerpt

The helicase UPF1 acts as the central essential factor in human nonsense-mediated mRNA decay (NMD) and is involved in various other mRNA degradation processes. Given its multifunctionality, distinguishing between mRNAs regulated directly and indirectly by UPF1 remains a critical challenge. We engineered two different conditional degron tags into endogenous UPF1 in human cell lines to probe the consequences of UPF1...

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Literature Corpus work
2e8ee9e8-4017-5cd0-b091-c8304e16f591
DOI
10.1101/2024.03.04.583328
Open publication

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Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated transcriptome in human cellsDOI 10.1101/2024.03.04.583328
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