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No observable guide-RNA-independent off-target mutation induced by prime editor

2021-04-09

Abstract excerpt

<h4>ABSTRACT</h4> Prime editor (PE) has been recently developed to induce efficient and precise ontarget editing, whereas its guide RNA (gRNA)-independent off-target effects remain unknown. Here, we used whole-genome and whole-transcriptome sequencing to determine gRNA-independent off-target mutations in cells expanded from single colonies, in which PE generated precise editing at on-target sites. We found that P...

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Identifiers and source

Literature Corpus work
28b09fd1-1bdb-5e78-b104-77585bcc3a94
DOI
10.1101/2021.04.09.439109
Open publication

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No observable guide-RNA-independent off-target mutation induced by prime editorDOI 10.1101/2021.04.09.439109
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