SynDesign: web-based prime editing guide RNA design and evaluation tool for saturation genome editing.

Abstract excerpt

Saturation genome editing (SGE) enables in-depth functional evaluation of disease-associated genes and variants by generating all possible single nucleotide variants (SNVs) within a given coding region. Although prime editing can be employed for inducing these SNVs, designing efficient prime editing guide RNAs (pegRNAs) can be challenging and time-consuming. Here, we present SynDesign, an easy-to-use webtool for...

Topics

• Gene Editing
• RNA, Guide, CRISPR-Cas Systems
• Humans
• Internet
• Software
• CRISPR-Cas Systems
• Mutation
• Polymorphism, Single Nucleotide