Back to search

Article

Cross-strain transferability of CRISPRi systems and design rules from laboratory to clinical <i>Escherichia coli</i> strains

2026-01-29

Abstract excerpt

CRISPR interference (CRISPRi) has emerged as a versatile approach for targeted gene repression in many organisms, including microbes and bacteria, due to the simple design of sequence-specific transcriptional silencing of gene expression. However, the strain-specific effects on repression efficiency and the host when translating a CRISPRi system from a laboratory strain to non-model strains are not well understood...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
243f8e96-dc8a-5bc6-8fad-d4a621d951f1
DOI
10.64898/2026.01.28.702340
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Cross-strain transferability of CRISPRi systems and design rules from laboratory to clinical <i>Escherichia coli</i> strainsDOI 10.64898/2026.01.28.702340
Multiplex CRISPRi-Cas9 silencing of planktonic and stage-specific biofilm genes in <i>Enterococcus faecalis</i>Semantic proximity
Mobile-CRISPRi: Enabling Genetic Analysis of Diverse BacteriaSemantic proximity
A broad-host-range CRISPRi toolkit for silencing gene expression in <i>Burkholderia</i>Semantic proximity
Use of CRISPR interference for efficient and rapid gene inactivation in <i>Fusobacterium nucleatum</i>Semantic proximity
A transferrable and integrative type I-F Cascade for heterologous genome editing and transcription modulationSemantic proximity
Awakening prime editing for precision engineering of probiotic <i>Escherichia coli</i> Nissle 1917Semantic proximity
CRISPRi-Driven Genetic Screening for Designing Novel Microbial Phenotypes.Semantic proximity
Harnessing CRISPR interference to resensitize laboratory strains and clinical isolates to last resort antibiotics.Semantic proximity
Engineering probiotic <i>Escherichia coli</i> Nissle 1917 to block transfer of multiple antibiotic resistance genes by exploiting a type I CRISPR-Cas systemSemantic proximity
Inducible CRISPRi-based operon silencing and selective <i>in trans</i> gene complementation in <i>Borrelia burgdorferi</i>Semantic proximity
Characterizing the activity of abundant, diverse and active CRISPR-Cas systems in lactobacilli.Semantic proximity
A duplexed CRISPRi-based approach to predict synergistic antibiotic targets in <i>Escherichia coli</i>Semantic proximity
Select a neighboring publication to make it the new centre.