Article

A duplexed CRISPRi-based approach to predict synergistic antibiotic targets in <i>Escherichia coli</i>

2024-05-16

Abstract excerpt

Co-repressing drug targets with CRISPR interference (CRISPRi) has the potential to predict both synergistic and additive drug combinations. In a screen experiment with Escherichia coli , pairwise gene co-repression assays correctly predicted the interaction type observed for 36 antibiotic pairs in checkerboard assays. Furthermore, we used this approach to identify promising synergistic interactions with a gene cu...

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Literature Corpus work: 22efb744-9bbf-5fa2-84e9-61378adfc69c
DOI: 10.1101/2024.05.15.594409

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