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Article

Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoids.

Life science alliance - 1 Oct 2021

Geurts Maarten H, de Poel Eyleen, Pleguezuelos-Manzano Cayetano, Oka Rurika, Carrillo Léo, Andersson-Rolf Amanda, Boretto Matteo, Brunsveld Jesse E, van Boxtel Ruben, Beekman Jeffrey M, Clevers Hans

Abstract excerpt

Prime editing is a recently reported genome editing tool using a nickase-cas9 fused to a reverse transcriptase that directly synthesizes the desired edit at the target site. Here, we explore the use of prime editing in human organoids. Common TP53 mutations can be correctly modeled in human adult stem cell-derived colonic organoids with efficiencies up to 25% and up to 97% in hepatocyte organoids. Next, we...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34373320
DOI: 10.26508/lsa.202000940
PMCID: PMC8356249

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