Article
Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice.
Nature communications - 9 Apr 2021
Liu Pengpeng, Liang Shun-Qing, Zheng Chunwei, Mintzer Esther, Zhao Yan G, Ponnienselvan Karthikeyan, Mir Aamir, Sontheimer Erik J, Gao Guangping, Flotte Terence R, Wolfe Scot A, Xue Wen
Abstract excerpt
Prime editors (PEs) mediate genome modification without utilizing double-stranded DNA breaks or exogenous donor DNA as a template. PEs facilitate nucleotide substitutions or local insertions or deletions within the genome based on the template sequence encoded within the prime editing guide RNA (pegRNA). However, the efficacy of prime editing in adult mice has not been established. Here we report an NLS-optimized...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.