Article

Evaluating CRISPR-based Prime Editing for cancer modeling and CFTR repair in intestinal organoids

2020-10-06

Abstract excerpt

Prime editing is a recently reported genome editing tool employing a nickase-cas9 fused to a reverse transcriptase that directly synthesizes the desired edit at the target site. The technique holds great promise for clinical application due to its versatility. Here, we explore the use of prime editing in human intestinal organoids. Common TP53 mutations were modeled in human adult stem cell with notable efficiency...

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Literature Corpus work: 609df4f8-3225-5dca-8888-f35324ae9381
DOI: 10.1101/2020.10.05.325837

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