Article

Transcription factors ERα and Sox2 have differing multiphasic DNA and RNA binding mechanisms

2024-03-19

Abstract excerpt

<h4>ABSTRACT</h4> Many transcription factors (TFs) have been shown to bind RNA, leading to open questions regarding the mechanism(s) of this RNA binding and its role in regulating TF activities. Here we use biophysical assays to interrogate the k on , k off , and K d for DNA and RNA binding of two model human transcription factors, ERα and Sox2. Unexpectedly, we found that both proteins exhibited multiphasic n...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 20c1c7b8-2c61-58a0-89ff-bb34683a0783
DOI: 10.1101/2024.03.18.585577

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Transcription factors ERα and Sox2 have differing multiphasic DNA and RNA binding mechanismsDOI 10.1101/2024.03.18.585577Read

SRYand architectural gene regulation: the kinetic stability of a bent protein-DNA complex can regulate its transcriptional potency.Semantic proximity Read

HIF1α-AS1 is a DNA:DNA:RNA triplex-forming lncRNA interacting with the HUSH complexSemantic proximity Read

DNA binding redistributes activation domain ensemble and accessibility in pioneer factor Sox2Semantic proximity Read

Tethered-hopping model for protein-DNA binding and unbinding based on Sox2-Oct1-Hoxb1 ternary complex simulations.Semantic proximity Read

Interactions with single-stranded and double-stranded DNA-binding factors and alternative promoter conformation upon transcriptional activation of the Htf9-a/RanBP1 and Htf9-c genes.Semantic proximity Read

Modulation of Nur77-DNA Interactions by the Glucocorticoid ReceptorSemantic proximity Read

Deciphering the Sox-Oct partner code by quantitative cooperativity measurements.Semantic proximity Read

RNA polymerase II initiation factors show different dynamic behaviour upon induced transcription in live cellsSemantic proximity Read

Role of promoter DNA sequence variations on the binding of EGR1 transcription factor.Semantic proximity Read

Rewiring of transcription factor binding in differentiating human embryonic stem cells is constrained by DNA sequence repeat symmetrySemantic proximity Read

Histone chaperone FACT action during transcription through chromatin by RNA polymerase II.Semantic proximity Read

Effective <i>in vivo</i> binding energy landscape illustrates kinetic stability of RBPJ-DNA bindingSemantic proximity Read