Article
Deciphering the Sox-Oct partner code by quantitative cooperativity measurements.
Nucleic acids research - 1 Jun 2012
Ng Calista K L, Li Noel X, Chee Sheena, Prabhakar Shyam, Kolatkar Prasanna R, Jauch Ralf
Abstract excerpt
Several Sox-Oct transcription factor (TF) combinations have been shown to cooperate on diverse enhancers to determine cell fates. Here, we developed a method to quantify biochemically the Sox-Oct cooperation and assessed the pairing of the high-mobility group (HMG) domains of 11 Sox TFs with Oct4 on a series of composite DNA elements. This way, we clustered Sox proteins according to their dimerization preferences...
Topics
- Amino Acid Sequence
- Animals
- Binding, Competitive
- DNA
- Dimerization
- Mice
- Molecular Sequence Data
- Mutation
- Octamer Transcription Factor-3
- Protein Binding
- Protein Interaction Domains and Motifs
- SOX Transcription Factors
- Sequence Alignment