Enhancing hypercompact CasΦ2 activity through EPICA.2, an optimized eukaryotic directed evolution platform

Abstract excerpt

Compact Cas nucleases offer advantages over the widely used SpCas9 due to their smaller size, which enables more efficient delivery for in vivo applications. Among these, the phage-encoded CasΦ2 (Cas12j2) is highly promising due to its relaxed PAM requirement (5′-TTN-3′) and compact size (757 aa); however, its translational potential is limited by low editing activity. To enhance the efficacy of CasΦ2, we optimize...

Topics

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• Adenine
• Adenosine Deaminase
• CRISPR-Associated Protein 9
• CRISPR-Cas Systems
• Gene Editing
• Genome
• HEK293 Cells
• Mutation

Identifiers and source

Literature Corpus work

1994df4d-ef77-5fde-8d06-4b32dd2202a2

DOI

10.64898/2026.08.12.744198