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Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells

2025-12-11

Abstract excerpt

<h4>ABSTRACT</h4> Engineering CRISPR-Cas systems for improved or altered function is central to both research and therapeutic applications. Unfortunately most optimization, especially directed evolution in bacterial hosts, fails to capture the functional requirements of the complex mammalian cellular milieu, where activity is usually required. Robust strategies to enable continuous directed evolution of genome-ta...

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Identifiers and source

Literature Corpus work
c98a57a1-6d69-5c41-9617-11eb7f7a5a77
DOI
10.64898/2025.12.11.693673
Open publication

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Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cellsDOI 10.64898/2025.12.11.693673
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