Article
Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells.
Proceedings of the National Academy of Sciences of the United States of America - 2 Jun 2026
Sabol Andrew L, Mengiste Amanuella A, Singh Prashant, Sreekanth Vedagopuram, Hendel Samuel J, Tran Minh Thuan Nguyen, Barybin Anton M, Chaudhary Santosh, Harris Ra'Mal M, Liivak Kristi E, Severance Zachary C, Locicero Cale M, Kailass Karishma, Lee Chaiheon, Xu Lucy Qinghua, Butty Vincent L, Choudhary Amit, Shoulders Matthew D
Abstract excerpt
Engineering CRISPR-Cas systems for improved or altered function is critical to both research and therapeutic applications. Unfortunately, most optimization, especially directed evolution in bacterial hosts, fails to capture the functional requirements of the complex mammalian cellular milieu, where activity is usually required. Robust strategies to enable continuous directed evolution of genome-targeting agents...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.