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Article

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity.

Nature biotechnology - 1 Jul 2020

Richter Michelle F, Zhao Kevin T, Eton Elliot, Lapinaite Audrone, Newby Gregory A, Thuronyi B W, Wilson Christopher, Koblan Luke W, Zeng Jing, Bauer Daniel E, Doudna Jennifer A, Liu David R

Abstract excerpt

Applications of adenine base editors (ABEs) have been constrained by the limited compatibility of the deoxyadenosine deaminase component with Cas homologs other than SpCas9. We evolved the deaminase component of ABE7.10 using phage-assisted non-continuous and continuous evolution (PANCE and PACE), which resulted in ABE8e. ABE8e contains eight additional mutations that increase activity (kapp) 590-fold compared...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32433547
DOI: 10.1038/s41587-020-0453-z
PMCID: PMC7357821

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