Article
Temperature-boosted PAM-less activation of CRISPR-Cas12a combined with selective inhibitors enhances detection of SNVs with VAFs below 0.01.
Talanta - 15 Aug 2023
Chen Kena, Dai Ling, Zhao Jie, Deng Mengjun, Song Lin, Bai Dan, Wu You, Zhou Xi, Yang Yujun, Yang Shuangshuang, Zhao Lin, Chen Xueping, Xie Guoming, Li Junjie
Abstract excerpt
The precise identification of rare single nucleotide variations (SNVs) concomitant with excess wild-type DNA is a valuable method for minimally invasive disease diagnosis and early prediction of drug responsiveness. Selective enrichment of mutant variants via strand displacement reaction offers an ideal approach of SNVs analysis but fails to differentiate wildtype from mutants with variant allele fraction...
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