Article
Detection of low-frequency mutations in clinical samples by increasing mutation abundance via the excision of wild-type sequences.
Nature biomedical engineering - 1 Dec 2023
Chen Wei, Xu Haiqi, Dai Shenbin, Wang Jiayu, Yang Ziyu, Jin Yuewen, Zou Mengbing, Xiao Xianjin, Wu Tongbo, Yan Wei, Zhang Bin, Lin Zhimiao, Zhao Meiping
Abstract excerpt
The efficiency of DNA-enrichment techniques is often insufficient to detect mutations that occur at low frequencies. Here we report a DNA-excision method for the detection of low-frequency mutations in genomic DNA and in circulating cell-free DNA at single-nucleotide resolution. The method is based on a competitive DNA-binding-and-digestion mechanism, effected by deoxyribonuclease I (DNase) guided by...
Topics
- Humans
- Mutation
- Neoplasms
- Polymerase Chain Reaction
- DNA
- Nucleotides